nextdenovo

nextdenovo 已适配多瑙调度器,由于计算节点目前没有安装 dsub 提交命令,因此需要在计算节点运行 nextdenovo 程序。

$ module load arm/nextdenovo/2.5.2
$ nohup nextDenovo run.cfg &
run.cfg 如下
[General]
job_type = donau
job_prefix = nextDenovo
task = all # 'all', 'correct', 'assemble'
rewrite = yes # yes/no
rerun = 3
parallel_jobs = 200
input_type = raw
input_fofn = input.fofn
read_type = ont
workdir = 01_rundir

[correct_option]
read_cutoff = 1k
genome_size = 650m
blocksize = 3g
pa_correction = 250
sort_options = -m 20g -t 8 -k 40
minimap2_options_raw = -x ava-ont -t 8
correction_options = -p 8

[assemble_option]
random_round = 20
minimap2_options_cns = -x ava-ont -t 8 -k17 -w17
nextgraph_options = -a 1
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